|
KMID : 0376219810180020373
|
|
Chonnam Medical Journal
1981 Volume.18 No. 2 p.373 ~ p.391
|
|
|
The Effect of BCG on the Morphological Alteration of RES Submitted to Zymosan or Methylcellulose
|
|
|
|
|
Abstract
|
|
|
Although BCG is the most widely used immunostimulatory agent in the iuununotherapy of malignant diseases, only limited information is available about BCG-induced morphological alteration of RES. It has been well known that though both zymosan and methylcellulose are stimulants to the reticuloendothelial cell proliferation, zymosan stimulates the phagocytic activity, while the activity is depressed by methylcellulose.
The purpose of this study is to determine the effect of BCG on the morphological alteration of RES submitted to methylcellulose or zymosan.
Experimental groups are divided into the following; BCG-treated, zymosan-treated, methylcellulose-treated, BCG-zymosan-treated, and BCGmethylcellulose-treated. The experimental rats of each group were sacrificed at 4, 8, 12 and 16 weeks intervals, and the liver, spleen and lung are studied by the histological and enzyme histochemical methods.
The results are as follows.
1. BCG-treated group showed systemic proliferation of macrophages and multifocal infiltration of pyroninophilic lymphocytes involving the portal areas of the liver, the white and red splenic pulps, and the bronchial lymph nodes. These reaetigns reached a peak at 4th and 8th weeks The BBC granuloma was observed only in the lung at 12th week. Increased activity of acid phosphatase was noted in the liver, spleen and lung.
2. Zymosan-treated group showed systemic proliferation of macrophages forming numerous nodules, in which phagocytosed zymosan was contained. The involved sites were the Kupffer cells of the liver, the marginal zone and red pulp of the spleen, the alveolar walls of the lung, and the bronchial lymph nodes. A slight increase of the acid phosphatase activity was observed in all organs tested.
3. In the met hycellulose -treated group, diffuse proliferation of macrophage was observed in the liver and lung, while the nodules containing phagocytosed methycellulose were marked only at the marginal zone and red pulp of the spleen. No significant changes of the enzyme activities tested were noted.
4. In the BCG and zymosan-treated group, the cellular proliferation and the phagocytic nodule formation seemed to be more pronounced than those of BCG-treated or zymosan-treated groups. Also there was marked elevation in the activities of the acid phosphatase, alkaline phosphatase and nonspecific esterase.
5. In the BCG and methylcellulose-treated group, the proliferation of macrophages, phagocytic nodule formation in both spleen and lung, as well as the increase in all enzyme activities were more marked than those of methylcellulose -treated group.
From these results, it may be concluded that BCG enhance the phagocytic function of the reticuloendothelial cells submitted to zymosan or methylcellulose.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|